Metaproteomics

DESCRIPTION
SPECIFICATION
RESOURCES

Project Workflow

DDA analysis can be carried out if there are no suitable library construction samples:
  • Protein extraction
  • Protein digestion
  • Individual samples
  • LC-MS/MS (DDA)
DIA analysis can be carried out if there are suitable library construction samples:

References


[1] Kleiner M. Metaproteomics: Much More than Measuring Gene Expression in Microbial Communities. mSystems. 2019 May-Jun; 4(3): e00115-19.

Mass Spectrometry Service Specification

Metaproteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF/HF-X and Orbitrap Fusion Lumos).
  • Sample Preparation and Services

    • The QC analysis of enzymatic hydrolysis is strictly controlled by LC-MS
    • DDA-Spectral Library generated using HPLC technology and sample fractionation


  • Quality Standard

    • Summary includes all methods and data analysis
    • Reports provided in Excel or PDF format, RAW files available upon request


  • Turn Around Time

    • Typical 4-5 weeks from sample QC acceptance to data report delivery


Data Analysis

DATA ANALYSIS
  • Data analysis and validation performed with Mascot
  • Protein Analysis
  • Species analysis
  • Diversity analysis
  • Function Analysis
  • Correlation Analysis


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