Metabolome + Metagenome / 16S

DESCRIPTION
SPECIFICATION
RESOURCES

Service Description

BGI offers multi-omics services to look across genomics, transcriptomics, epigenomics, proteomics and metabolomics, with the flexibility to customize solutions that meet your specific needs. All projects are supported by an advanced bioinformatics infrastructure.

Metabolome + Metagenome / 16S Analysis

In recent years, research on gut related diseases has developed rapidly and it is now thought that nearly 90% of diseases may be related to gut microbiota health. Metagenome/16S + metabolome correlation analysis enables researchers to establish a correlation model between host metabolism and gut microbiota and explore the causal relationships between microbes and disease1-6.

Project Workflow


Note: Stools and intestinal contents samples are recommended for Metagenome/16S services; blood samples (serum, plasma) are recommended for Metabolome services. No biological correlation analysis for the differential metabolites and the differential species.

References

[1] Mendelian randomization analyses support causal relationships between blood metabolites and the gut microbiome. Nature Genetics. 2022 Jan; 54(1): 52-61. (BGI's Publication)

[2] Endosymbionts reduce microbiome diversity and modify host metabolism and fecundity in the planthopper Sogatella furcifera. mSystems. 2022 Apr 26; 7(2): e0151621. (BGI's Customer)

[3] Life history recorded in the vagino-cervical microbiome along with multi-omics. Genomics Proteomics Bioinformatics. 2021 Jun 9: S1672-0229(21)00099-1. (BGI's Publication)

[4] Metagenome-wide association of gut microbiome features for schizophrenia. Nat Commun. 2020 Mar 31;11(1):1612. (BGI's Publication)

[5] Transplantation of microbiota from drug-free patients with schizophrenia causes schizophrenia-like abnormal behaviors and dysregulated kynurenine metabolism in mice. Mol Psychiatry. 2020 Nov;25(11):2905-2918. (BGI's Publication)

[6] Gut microbiome-related effects of berberine and probiotics on type 2 diabetes. Nat Commun. 2020 Oct 6;11(1):5015. (BGI's Publication)

Mass Spectrometry Service Specification

16S/Metagenome sequencing platform: 16S/Metagenome services. Metabolome platform: Untargeted/Targeted Metabolomics and Lipidomics services.

  • Quality Standard

    • Summary includes data analysis
    • Reports provided in PDF format, RAW files available upon request

  • Turn Around Time

    • Typical 2 weeks from LC-MS/MS raw data acceptance to data report delivery

Sample Requirements

Sample selection for 16S/Metagenome and Metabolome should be as consistent as possible.
INTEGRATIVE ANALYSISBIOLOGICAL DUPLICATES WHEN 2 GROUPSBIOLOGICAL DUPLICATES WHEN ≥ 3 GROUPS
Metagenome + metabolome≥ 8≥ 6
16S+ metabolome≥ 8≥ 4
Clinical samples≥ 30≥ 30

Data Analysis

METABOLOME + METAGENOME / 16S CORRELATION ANALYSIS
  • Univariate correlation
  • Unsupervised model correlation
  • Supervised model correlation
  • Biological function correlation

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