Protein Identification

DESCRIPTION
SPECIFICATION
RESOURCES

Service Description

Modern liquid chromatography and mass spectrometry technology provides a means to routinely perform non-targeted protein identification¹. BGI can help simplify the challenge of protein identification for your project.

Project Workflow

In-Gel Identification

We can identify proteins isolated in gel bands or spots. Our service includes destaining, in-gel digestion, nano-flow LC-MS/MS, and peptide spectral match data analysis.
  • In-gel digestion
  • LC-MS/MS ANALYSIS
  • Data analysis

In-Solution Identification

Our in-solution protein identification services can be used for applications such as monitoring recombinant protein expression and affinity purification systems.
  • In-solution digestion
  • LC-MS/MS analysis
  • Data analysis

References

[1]. Baldwin MA. Protein identification by mass spectrometry: issues to be considered. Mol Cell Proteomics. 2004 Jan;3(1):1-9. Epub 2003 Nov 6. Review. PubMed PMID: 14608001

Mass Spectrometry Service Specification

Protein Identification services are performed using nano-flow liquid chromatography and high resolution Orbitrap mass spectrometry.

  • Sample Preparation and Services

    • Digestion performed using sequencing-grade trypsin
    • 60 min nano-flow LC-MS/MS using a Q Exactive HF-X Orbitrap mass spectrometer

  • Mass Spec Services Quality Standard

    • Summary including all methods and data analysis
    • Reports provided in Excel or PDF format, RAW files available upon request

  • Turn Around Time

    • Typical 2-3 weeks from sample QC acceptance to data report delivery
    • Prime Expedited Services with 1 week Turn Around time is available

Sample Requirements

We accept protein samples in solution or gel format. For complex in-solution samples we can provide electrophoresis services, including preparative SDS-PAGE and analytical staining.
Sample typeAmount and ConcentrationMinimum sample volume
Protein sample in liquid solution or gel band/spotRecommended 100 µg; 2 µg/µL or above10 µL

Data Analysis

ADVANCED BIOINFORMATICS
  • Data analysis and validation performed with SEQUEST or Mascot
  • Top protein identifications, peptide spectral match count, and sequence coverage provided
  • Up to 30 proteins ranked by peptide spectral match count

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