Untargeted Metabolomics


Service Description

BGI has extensive experience in the field of untargeted metabolomics with developed reliable workflows using market leading technologies and an advanced bioinformatics infrastructure.

Untargeted Metabolomics

By applying state-of-the-art LC-MS/MS systems and techniques, BGI offers validated workflows of untargeted metabolomics to support biomedical and biotechnology applications¹. Our Untargeted Metabolomics services are designed to obtain a metabolite profile and screen for differentially-expressed molecules in the sample.

Project Workflow


[1] Liu R., et al., Gut microbiome and serum metabolome alterations in obesity and after weight-loss intervention. Nat Med, 2017. 23(7): p. 859-868.

Mass Spectrometry Service Specification

Untargeted Metabolomics services are performed using Waters ACQUITY UPLC and cutting-edge mass spectrometer (Q Exactive HF/HF-X and Q-TOF 5600).
  • Sample Preparation and Services

    • Increase metabolome depth of coverage using both ACQUITY UPLC BEH C18 and Amide column
    • (+) and (-) ESI detection by a Q Exactive HF/HF-X mass spectrometer

  • Quality Standard

    • Summary includes all methods and data analysis
    • Reports provided in Excel or PDF format, RAW files available upon request

  • Turn Around Time

    • Typical 5-7 weeks after sample receipt for raw data delivery

Sample Requirements

We accept metabolite samples in a variety of formats. Contact your BGI account representative for details for your samples.
Serum, plasma≥ 300 µL≥ 100 µL
Urine≥ 300 µL≥ 100 µL
Animal and clinical tissues≥ 200 mg≥ 25 mg
Feces and intestinal contents≥ 200 mg≥ 25 mg
Microorganism≥ 200 mg≥ 25 mg
Culture medium, fermentation medium≥ 1 mL≥ 100 µL
Milk≥ 1 mL≥ 100 µL
Other body fluids (amniotic fluid, saliva, hemolymph, cerebrospinal fluid, etc.)≥ 300 µL≥ 100 µL
  • Data analysis performed with metaX1 and BGI library
  • T-test, PCA, PLS-DA, OPLS-DA for differential metabolite identification
  • Cluster analysis
  • Metabolite pathway annotation
  • ROC analysis
  • Metabolite correlation analysis