Metagenomic Sequencing

Metagenomic Sequencing

Service Description

Over 99% of natural microorganisms can not be isolated and cultured clonally. Traditional isolation and culture-dependent methods have limited the study of microorganisms in their natural environment. Metagenomic studies of genetic material that is directly recovered from environmental samples, have benefited greatly from advanced NGS technology as a method for the exploration of microbial biodiversity.

BGI’s metagenomics survey service applies whole genome shotgun sequencing of DNA isolated from environmental samples, with the advantages of high throughput and high coverage. It can provide information not only on species composition and abundance, but also on functional genes, gene differences between samples, metabolic pathways and gene resource mining for bioactive products.

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

  • Sample QC
  • Library QC
  • Sequencing QC
  • Data QC
  • Sample Preparation
  • Library Preparation
  • Sequencing
  • Raw Data Output
  • Bioinformatics Analysis

How to order

Request a Quote

Send us a no obligation request for quote and our dedicated sales team will reply to you within 24 hours.

Create an Online myBGI Account

Create a myBGI account to view all our sample and shipping documentation and to manage your project moving forwards. It's quick, free and easy!

Sequencing Service Specification

BGI Metagenomic Survey Sequencing services are executed with the Illumina HiSeq sequencing system.

  • Sample Preparation and Services

    Sample Preparation and Services

    • 150bp paired end sequencing
    • Raw data, standard and customized data analysis
    • Cloud-based data storage and delivery system
  • Sequencing Quality Standard

    Sequencing Quality Standard

    • Q20≥83%
  • Turn Around Time

    Turn Around Time

    • Typical 40 working days from sample QC acceptance to filtered raw data availability
    • Expedited services are available, contact your local BGI specialist for details

Sample Requirements

We can process your exacted genomic DNA from a variety of environmental samples with the following general requirements:

DNA Mass and Concentration

Minimum Sample Volume

Regular Samples

Intact genomic DNA ≥ 1.5μg, Concentration ≥ 20 ng/μl

15 μl

Low Input Samples

Intact genomic DNA ≥ 200 ng, Concentration ≥ 2.5 ng/μl

15 μl

Data Analysis

In addition to raw data output, BGI offers a range of standard and customized bioinformatics pipelines for your whole genome sequencing project.

Standard Analysis

  • Filtering

  • Alignment

  • Metagenomic De Novo assembly

  • Non-redundant gene catalogue

  • Prophage transposable element prediction

  • Functional annotation based on KEGG, CAZy, eggNOG, ARDB

  • Species composition and diversity analysis

  • Quantitative and differential analysis of gene abundance

  • Principal component analysis

Customized analysis

Further customization of Bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative

Metagenomic Sequencing Resources

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.